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Sequencing

    Whole Metagenome Sequencing

    Metagenome sequencing is the sequencing of DNA extracted from microbial community from natural environment (without culturing) to reveal functional gene composition and physiological aspects.


    Applications

    1. Community diversity analysis, includes gene, genotype, species, phylogenetic, evolution, ecological niche, functional, morphological, structural etc

    2. Analyzing the change in microbial communities in varying conditions and time points

    3. Identification and genetic characterization of unknown microbes

    4. Identification of new genes and functional annotation with reference to environmental condition

    5. Establishing relationship among genes, mRNAs and proteins in microbial communities

    6. Establishing relationship among genes, mRNAs and proteins in microbial communities


    Sequencing Workflow

    1. DNA Extraction

    2. DNA quality check using Qubit and Gel Electrophoresis

    3. Preparation of the sequencing libraries

    4. Validation of the prepared library

    5. Sequencing (HiSeq/NextSeq/Pacbio)

    6. Bioinformatics Analysis