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Sequencing

    Small RNA Sequencing

    Small RNAs are non-coding RNA, ranging from 20 to 40 nucleotide in length, that play an important role in the regulation of gene expression in the cytoplasm and the nucleus through inducing both post- translational and transcriptional gene silencing.


    Applications

    1. Understanding the role of post-transcriptional regulation on phenotype

    2. Identification of novel gene and biomarker

    3. Development of personalized medicine

    4. Development of personalized medicine


    Sequencing Workflow

    1. RNA Extraction

    2. RNA quality check using Qubit and Bioanalyser

    3. Preparation of the sequencing libraries

    4. Validation of the prepared library

    5. Sequencing (MiSeq/NextSeq)

    6. Bioinformatics Analysis